Soil sample of Gopalpur soil series was taken to understand heavy metal effect on bacterial characterization. The soil was collected in the laboratory using a thermos flask. The sample was sieved and sorted properly and mixed throughly. Analytical-grade sulfate (SO₄²⁻) salts of zinc, copper, and cadmium were applied to designated pots, both individually and in combinations. The soil sample that received no metal treatment served as the control. These heavy metals (Cu, Cd & Zn) and their combinations were applied to the soil. 0 day, 15 days, 30 days, 45 days and 60 days were selected as incubation periods. The experiment was conducted using a Completely Randomized Design (CRD) with three replications. Combined applications of heavy metals are found more toxic to bacterial population than single application. The treatments, both Cd (3000ppm) and Cu+Cd+Zn (1000ppm+1000ppm+1000ppm), were found more toxic to both Gram- positive bacteria and Gram-negative bacteria. If we compare with Gram- positive bacteria, Gram-negative bacteria were found more resistant. Treatments, both Cd (3000ppm) and Cu+Cd+Zn (1000ppm+1000ppm+1000ppm), were more toxic to both non spore forming bacteria and spore forming bacteria. Here, spore forming bacteria were found more resistant. Treatment,Cu+Cd+Zn(1000ppm+1000ppm+1000ppm), was more toxic to both non capsulated bacteria and capsulated bacteria. We found capsulated bacteria bacteria as more resistant. Gram-positive bacteria, non spore forming bacteria, non capsulated bacteria were very sensitive whereas Gram-negative bacteria, spore forming bacteria and capsulated bacteria were available as resistant to heavy metals.