This study investigated the physicochemical properties, phytochemical composition, fatty acid profile, FT-IR functional group characterization, and cyanogenic glycoside content of Neocarya macrophylla (gingerbread plum) seed oil sourced from Kebbi State, north-western Nigeria. Oil was extracted by Soxhlet method with n-hexane and analyzed using AOCS/AOAC standard methods, qualitative phytochemical tests, Fourier-transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Physicochemical analysis yielded a saponification value of 153.33 mgKOH/g, iodine value of 33.07 gI2/100g, peroxide value of 45.5 meq/kg, free fatty acid content of 3.72%, and acid value of 13.41 mgKOH/g, classifying the oil as a non-drying oil suitable for soap and cosmetic manufacture. FT-IR spectroscopy confirmed key functional groups: O–H carboxylic acid stretching at 3475.84 cm⁻¹, ester carbonyl C=O at 1740.81 cm⁻¹, and C–O triacylglycerol vibrations at 721.4 cm⁻¹, consistent with a triacylglycerol-rich oil composition. Qualitative phytochemical screening confirmed alkaloids, tannins, flavonoids, saponins, steroids, and terpenoids; cardiac and cyanogenic glycosides were absent. GC-MS fatty acid profiling identified eleven fatty acids; oleic acid was predominant at 42.46%, followed by icosatetraenoate/arachidonic acid (17.67%) and eicosatrienoic acid, palmitic, stearic, and erucic acids. The MUFA/PUFA ratio of ~1.31 indicates favourable oxidative stability. Collectively, these results confirm the multi-sector industrial potential of N. macrophylla seed oil for soap, cosmetics, pharmaceuticals, and nutraceutical applications.